rat anti cd206  (Bio-Rad)

Journal: iScience

Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination

doi: 10.1016/j.isci.2026.114718

Figure Lengend Snippet: Aurkb loss transiently elevates CD68 in homeostatic microglia but compromises its upregulation in an LPS-induced inflammation model (A–D) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates (8-week-old) were i.p. injected with TAM for 5 consecutive days, followed by tissue collection at 1-month and 3-month post TAM induction. Representative immunofluorescence and quantification of CD68 in microglia at (A and B) 1-month and (C and D) 3-month post-TAM induction ( n = 5 mice per genotype per time point, Scale bars: 50 μm). (E–H) Neonatal Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates were i.p. injected with TAM for 3 consecutive days at P1-P3, followed by tissue collection at P13. Representative immunofluorescence and quantification of (E and F) CD68 and (G and H) CD206 in microglia at P13 ( n = 6 mice per genotype, Scale bars: 50 μm). (I and J) Representative immunofluorescence and quantification of CD68 in microglia from adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates ( n = 5 mice per genotype, Scale bars: 50 μm). The representative immunofluorescence image of the Cx3cr1 Cre/+ Aurkb fl/fl group is shared in C. (K and L) Adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates were i.p. injected with LPS (1 mg/kg) and sacrificed at 48 h post LPS administration ( n = 5 mice per genotype, Scale bars: 50 μm). Data are presented as the mean ± SD. Two-tailed unpaired t-tests in (B, D, J, and I); two-way ANOVA with Bonferroni multiple comparisons test in (F, H); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant compared with the Aurkb fl/fl group. See also and .

Article Snippet: The following primary antibodies were used: rabbit anti-Iba-1 antibody (1:500, Wako, Cat: 019–19741), mouse anti-Iba-1 antibody (1:400, Abcam, Cat: ab283319), rat anti-CD206 antibody (1:200, Bio-Rad, Cat: MCA2235), rat anti-BrdU antibody (1:400, Abcam, Cat: ab6326), mouse anti-phospho-Histone H3 (Ser10) antibody (1:200, Cell Signaling Technology, Cat: 9706S), mouse anti-APC (1:100, CC-1, Merck, Cat: OP80), rat anti-myelin basic protein (Mbp) monoclonal antibody (1:500, Abcam, Cat: ab7349), rabbit anti-degraded myelin basic protein (dMbp) antibody (1:2000, Millipore Sigma, Cat: AB5864), rabbit anti-Olig2 antibody (1:500, Proteintech, Cat: 13999-1-AP) and rat anti-CD68 antibody (1:500, Abcam, Cat: ab53444).

Techniques: Injection, Immunofluorescence, Two Tailed Test

Journal: iScience

Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination

doi: 10.1016/j.isci.2026.114718

Figure Lengend Snippet: Aurkb loss transiently elevates CD68 in homeostatic microglia but compromises its upregulation in an LPS-induced inflammation model (A–D) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates (8-week-old) were i.p. injected with TAM for 5 consecutive days, followed by tissue collection at 1-month and 3-month post TAM induction. Representative immunofluorescence and quantification of CD68 in microglia at (A and B) 1-month and (C and D) 3-month post-TAM induction ( n = 5 mice per genotype per time point, Scale bars: 50 μm). (E–H) Neonatal Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates were i.p. injected with TAM for 3 consecutive days at P1-P3, followed by tissue collection at P13. Representative immunofluorescence and quantification of (E and F) CD68 and (G and H) CD206 in microglia at P13 ( n = 6 mice per genotype, Scale bars: 50 μm). (I and J) Representative immunofluorescence and quantification of CD68 in microglia from adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates ( n = 5 mice per genotype, Scale bars: 50 μm). The representative immunofluorescence image of the Cx3cr1 Cre/+ Aurkb fl/fl group is shared in C. (K and L) Adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates were i.p. injected with LPS (1 mg/kg) and sacrificed at 48 h post LPS administration ( n = 5 mice per genotype, Scale bars: 50 μm). Data are presented as the mean ± SD. Two-tailed unpaired t-tests in (B, D, J, and I); two-way ANOVA with Bonferroni multiple comparisons test in (F, H); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant compared with the Aurkb fl/fl group. See also and .

Article Snippet: Rat anti-Mouse CD206 Monoclonal antibody, clone MR5D3 , Bio-Rad , Cat# MCA2235, RRID: AB_324622.

Techniques: Injection, Immunofluorescence, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD

doi: 10.1016/j.xcrm.2025.102522

Figure Lengend Snippet: SA and GAA attenuated MASH progression (A) Schematic workflow. Wild-type (WT) mice were fed an LFD or GAN diet for 20 weeks ( n = 8/group). (B–D) Serum ALT, AST, and TG ( n = 8/group). (E) Representative H&E histology, oil red, and Sirius red staining of liver sections. Scale bar: 100 μm. (F) Liver-to-body weight ratio (Liver index) ( n = 8/group). (G) Hepatic triglycerides ( n = 8/group). (H) NAS ( n = 8/group). (I) Area fraction of oil red O staining (%) ( n = 8/group). (J) Area fraction of Sirius red staining (%) ( n = 8/group). (K) Representative immunofluorescent staining of CD11c and CD206 and immunohistochemical staining of Ly6G in liver sections. Scale bar: 100 μm. (L) Flow cytometry analysis of intrahepatic CD11b + F4/80 + cells ( n = 8/group). (M) Adgre1 (F4/80-coding gene) mRNA expression ( n = 8/group). (N) Ccl2 (MCP1-coding gene) mRNA expression ( n = 8/group). (O) Area fraction of Ly6G staining (%) ( n = 8/group). (P) Ly6g (Ly6G-coding gene) mRNA expression ( n = 8/group). (Q) Tnf (TNF-α-coding gene) mRNA expression ( n = 8/group). (R) Representative immunohistochemical staining of 4-HNE in liver sections. Scale bar: 100 μm. (S) Area fraction of 4-HNE staining (%) ( n = 8/group). (T) GSSG/GSH ratio of mice livers ( n = 8/group). Data are presented as the mean ± SEM. Unpaired Student’s t test was used between two groups. One-way ANOVA was used between multiple groups. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Rat anti Mouse CD206 antibody, clone MR5D3 , BioRad , Cat#MR5D3 ; RRID:AB_324449.

Techniques: Staining, Immunohistochemical staining, Flow Cytometry, Expressing